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biotinylated goat anti hil 33  (R&D Systems)


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    R&D Systems biotinylated goat anti hil 33
    Biotinylated Goat Anti Hil 33, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+hil+33/Human+IL-33+Biotinylated+Antibody/pm34930320-187-7-11
    Average 92 stars, based on 12 article reviews
    biotinylated goat anti hil 33 - by Bioz Stars, 2026-09
    92/100 stars

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    Incubation:

    Article Title: Interleukin 33/ST2 Axis Components Are Associated to Desmoplasia, a Metastasis-Related Factor in Colorectal Cancer
    Article Snippet: Then, they were heated in an EDTA-based buffer at pH 9.0 (Buffer EnVision Flex Antigen Retrieval, Dako, Carpinteria, CA, USA), using an electric pressure cooker for 3 min at 12–15 pounds/square inch at ~120°C, and cooled for 10 min before immunostaining. .. Then, all sections were incubated with 3% H 2 O 2 (blockade of endogenous peroxidases) for 10 min and subsequently incubated with goat anti-hIL-33 (R&D Systems, Minneapolis, MN, USA), goat anti-hST2 (R&D Systems, Minneapolis, MN, USA), mouse anti-α-SMA (Sigma–Aldrich, St. Louis, MO, USA) and mouse anti-E-cadherin (BD Biosciences, Franklin Lakes, NJ, USA) primary antibody for 30 min each. .. Tissue sections were incubated with a goat–rabbit IgG linker (for samples incubated with goat polyclonal antibodies) and then incubated with the secondary antibody–universal polymer (EnVision Flex-HRP, Dako, Carpinteria, CA, USA).



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    Comparison of <t>hIL-33</t> standard curves established using eBioscience hIL-33 platinum ELISA kit and hIL-33 provided with the kit (purple circle), in-house reduced hIL-33 (blue triangle), and oxidized hIL-33 (red inverted triangle). Data are means ± SD of triplicates. * = P < 0.05, ** = P < 0.01, ***P < 0.001
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    Comparison of <t>hIL-33</t> standard curves established using eBioscience hIL-33 platinum ELISA kit and hIL-33 provided with the kit (purple circle), in-house reduced hIL-33 (blue triangle), and oxidized hIL-33 (red inverted triangle). Data are means ± SD of triplicates. * = P < 0.05, ** = P < 0.01, ***P < 0.001
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    Comparison of hIL-33 standard curves established using eBioscience hIL-33 platinum ELISA kit and hIL-33 provided with the kit (purple circle), in-house reduced hIL-33 (blue triangle), and oxidized hIL-33 (red inverted triangle). Data are means ± SD of triplicates. * = P < 0.05, ** = P < 0.01, ***P < 0.001

    Journal: Journal of Translational Medicine

    Article Title: Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development

    doi: 10.1186/s12967-021-03189-3

    Figure Lengend Snippet: Comparison of hIL-33 standard curves established using eBioscience hIL-33 platinum ELISA kit and hIL-33 provided with the kit (purple circle), in-house reduced hIL-33 (blue triangle), and oxidized hIL-33 (red inverted triangle). Data are means ± SD of triplicates. * = P < 0.05, ** = P < 0.01, ***P < 0.001

    Article Snippet: C Schematic representation of total hIL-33 ELISA format: in-house rat anti-hIL-33 15.21C7 capture MAb (dark blue) and R&D System’s goat anti-hIL-33 PAb (orange), in-house reduced hIL-33 (purple) and oxidized hIL-33 (light purple) standards in the presence of hsST2 (gray), and R&D System’s biotinylated goat anti-hST2 (green) and goat anti-hIL-33 (orange) PAbs.

    Techniques: Comparison, Enzyme-linked Immunosorbent Assay

    Development of reduced and total (oxidized + reduced) hIL-33 ELISAs. A Schematic representation of reduced hIL-33 ELISA format: in-house rat anti-hIL-33 15.21C7 capture MAb (dark blue), in-house reduced hIL-33 standard (purple), and in-house biotinylated rat/human chimeric anti-hIL-33 3F10 detection MAb (light blue). B Comparison of standard curves obtained with reduced hIL-33 (blue), oxidized hIL-33 (red), reduced hIL-33 + 20 ng/mL hsST2 (light blue), reduced hIL-33 + 40 ng/mL hsST2 (green). C Schematic representation of total hIL-33 ELISA format: in-house rat anti-hIL-33 15.21C7 capture MAb (dark blue) and R&D System’s goat anti-hIL-33 PAb (orange), in-house reduced hIL-33 (purple) and oxidized hIL-33 (light purple) standards in the presence of hsST2 (gray), and R&D System’s biotinylated goat anti-hST2 (green) and goat anti-hIL-33 (orange) PAbs. D Comparison of reduced and oxidized hIL-33 standard curves in presence of 20 ng/mL hsST2. Data are representative of three experiments performed in duplicates. E Specificity of total hIL-33 ELISA determined by immunodepletion of reduced and oxidized hIL-33 in diluted human VH samples using beads conjugated to hIL-33 Ab. P = 0.0019. Data are means ± SD of duplicates

    Journal: Journal of Translational Medicine

    Article Title: Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development

    doi: 10.1186/s12967-021-03189-3

    Figure Lengend Snippet: Development of reduced and total (oxidized + reduced) hIL-33 ELISAs. A Schematic representation of reduced hIL-33 ELISA format: in-house rat anti-hIL-33 15.21C7 capture MAb (dark blue), in-house reduced hIL-33 standard (purple), and in-house biotinylated rat/human chimeric anti-hIL-33 3F10 detection MAb (light blue). B Comparison of standard curves obtained with reduced hIL-33 (blue), oxidized hIL-33 (red), reduced hIL-33 + 20 ng/mL hsST2 (light blue), reduced hIL-33 + 40 ng/mL hsST2 (green). C Schematic representation of total hIL-33 ELISA format: in-house rat anti-hIL-33 15.21C7 capture MAb (dark blue) and R&D System’s goat anti-hIL-33 PAb (orange), in-house reduced hIL-33 (purple) and oxidized hIL-33 (light purple) standards in the presence of hsST2 (gray), and R&D System’s biotinylated goat anti-hST2 (green) and goat anti-hIL-33 (orange) PAbs. D Comparison of reduced and oxidized hIL-33 standard curves in presence of 20 ng/mL hsST2. Data are representative of three experiments performed in duplicates. E Specificity of total hIL-33 ELISA determined by immunodepletion of reduced and oxidized hIL-33 in diluted human VH samples using beads conjugated to hIL-33 Ab. P = 0.0019. Data are means ± SD of duplicates

    Article Snippet: C Schematic representation of total hIL-33 ELISA format: in-house rat anti-hIL-33 15.21C7 capture MAb (dark blue) and R&D System’s goat anti-hIL-33 PAb (orange), in-house reduced hIL-33 (purple) and oxidized hIL-33 (light purple) standards in the presence of hsST2 (gray), and R&D System’s biotinylated goat anti-hST2 (green) and goat anti-hIL-33 (orange) PAbs.

    Techniques: Enzyme-linked Immunosorbent Assay, Comparison, Immunodepletion

    Development and specificity of 384-well reduced hIL-33 immuno-PCR (iPCR) assay. A Schematic representation of reduced hIL-33 iPCR assay format: biotinylated rat/human chimeric anti-hIL-33 3F10 capture MAb (light blue), reduced hIL-33 standard (purple), and 55-mer DNA-labeled rat IgG2a anti-hIL-33 15.21C7 detection MAb (dark blue). B Reduced hIL-33 standard curve obtained using iPCR in sample buffer. Data are means ± SD of 6 independent standard curves, each performed with 4 replicates. C Specificity of reduced hIL-33 iPCR assay determined by immunodepletion of reduced hIL-33 in diluted human VH samples using beads conjugated to rat anti-reduced hIL-33 15.21C7 IgG2a (gray bars), control beads conjugated to rat anti-gp120 IgG2a (white bars) and sample buffer (black bars). P = 0.0004 (1:2 VH), P = < 0.0001 (1:4 VH). Data are means ± SD of triplicates

    Journal: Journal of Translational Medicine

    Article Title: Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development

    doi: 10.1186/s12967-021-03189-3

    Figure Lengend Snippet: Development and specificity of 384-well reduced hIL-33 immuno-PCR (iPCR) assay. A Schematic representation of reduced hIL-33 iPCR assay format: biotinylated rat/human chimeric anti-hIL-33 3F10 capture MAb (light blue), reduced hIL-33 standard (purple), and 55-mer DNA-labeled rat IgG2a anti-hIL-33 15.21C7 detection MAb (dark blue). B Reduced hIL-33 standard curve obtained using iPCR in sample buffer. Data are means ± SD of 6 independent standard curves, each performed with 4 replicates. C Specificity of reduced hIL-33 iPCR assay determined by immunodepletion of reduced hIL-33 in diluted human VH samples using beads conjugated to rat anti-reduced hIL-33 15.21C7 IgG2a (gray bars), control beads conjugated to rat anti-gp120 IgG2a (white bars) and sample buffer (black bars). P = 0.0004 (1:2 VH), P = < 0.0001 (1:4 VH). Data are means ± SD of triplicates

    Article Snippet: C Schematic representation of total hIL-33 ELISA format: in-house rat anti-hIL-33 15.21C7 capture MAb (dark blue) and R&D System’s goat anti-hIL-33 PAb (orange), in-house reduced hIL-33 (purple) and oxidized hIL-33 (light purple) standards in the presence of hsST2 (gray), and R&D System’s biotinylated goat anti-hST2 (green) and goat anti-hIL-33 (orange) PAbs.

    Techniques: Labeling, Immunodepletion, Control

    Reduced hIL-33 concentrations in VH and AH samples measured by reduced hIL-33 iPCR assay. A Reduced hIL-33 concentrations of postmortem human VH samples from 17 control (blue circles) and 19 AMD (red circles) donors. P = 0.0155. B Reduced hIL-33 concentrations of postmortem human AH samples from ten control (blue circles) and nine AMD (red circles) donors. ns = no significance. Data are means of duplicates

    Journal: Journal of Translational Medicine

    Article Title: Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development

    doi: 10.1186/s12967-021-03189-3

    Figure Lengend Snippet: Reduced hIL-33 concentrations in VH and AH samples measured by reduced hIL-33 iPCR assay. A Reduced hIL-33 concentrations of postmortem human VH samples from 17 control (blue circles) and 19 AMD (red circles) donors. P = 0.0155. B Reduced hIL-33 concentrations of postmortem human AH samples from ten control (blue circles) and nine AMD (red circles) donors. ns = no significance. Data are means of duplicates

    Article Snippet: C Schematic representation of total hIL-33 ELISA format: in-house rat anti-hIL-33 15.21C7 capture MAb (dark blue) and R&D System’s goat anti-hIL-33 PAb (orange), in-house reduced hIL-33 (purple) and oxidized hIL-33 (light purple) standards in the presence of hsST2 (gray), and R&D System’s biotinylated goat anti-hST2 (green) and goat anti-hIL-33 (orange) PAbs.

    Techniques: Control